Biochemistry (Paper Code: 17010203)

 

    
Introduction and Importance of biochemistry in general and in particular to Agriculture & biotechnology, plant cell and cell ion constituents (biomolecules) chemistry of biomolecules. Their definition & functions. Carbohydrates: biologically important monosaccharides, chiral carbon, stereoisomerism, optical activity, mutarotation reactions of monosaccharides. Structure and function of oligio and polysaccharides. A brief account of some important carbohydrate metabolic. Pathways (Glycolysis, TCA cycle, HMP, Glyoxylate pathway Gluconeosenesis), Electron transport and oxidative phosphorylation.                                  

 Classification and functions of lipids: Saponifiable & non-saponifiable lipids. Structure and properties of common saturated, unsaturated and OH-fatty acids. Chemical properties of fatty acids, Triacyclelycerols and characterization of fats (saponification, saponifiable number, Iodine number, acetyl number, Acid number, Reichest Missed number), Rancidity, waxes, Compound Lipids (Phospholipids & glycolipids), oxidation of saturated fatty acids: B- oxidation Pathway 

Introductions functions, classification and structure of L- Amminoacids, peptide bond, classifications, proteins: their biological functions and structural organization (primary secondary, tertiary & quaternary), General and degradation reactions, amino acids, Urea cycle, enzymes: classification characteristics and factors affecting enzymes activity: coenzymes, prosthetic group.

Introduction, structure and functions of Nitrogen bases (Purifers & Pyrimidines) Nucleosides, Nucleotides, Common ribo and deoxyribio nucleotides, some important chemical and physical properties of nucleic acids, preliminary aspects of DNA replication, transcription and translation::

  

 

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Must know
photosynthesis, photorespiration, ammonia & Nitrate assimilation and symbiotic N2 fixation  

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 Desirable to Know
Steroids (structure and functions of cholesterols, ergosterol)

Plant hormones and second any metabolites their physiological roles

  

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 Nice to Know
Practical

Preparation of solutions, use of pH meter, color reactions of carbohydrates, Amino acids, proteins, lipids and nucleic acids: separation of sugars and amino acids by paper chromatography: Demonstration of simple enzymes assay.

  

 

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Lipids by TLC 5  
Preparation of solutions buffers, separation of sugars by paper chromatography  

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Syllabus books

1.       Nelson David and Cox Michael, 2009. Lehninger principles of biochemistry, 5th edn, WH freeman and company

2.       Voet, D., and Voet G.J., 2012. Princiuples of biochemistry, 4th edn, Wiley publisher

3.       Jain, J.L. 2004. Fundamentals of Biochemistry. 5th edn. Published by S.Chand and Company, New Delhi

4.       Conn, E.E and Stumpf, P.K. 1989. Outline of Biochemistry. Wiley Eastern Ltd. New Delhi.

5.       Buchanann B., and Gruissem W., and Jones R., 2002. Biochemistry and molecular biology of plants, Wiley-Blackwell publishers